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John J Lemasters, MD, PhD
Professor of Drug Discovery & Biomedical Sciences
College of Pharmacy
MUSC

Email: lemaste@musc.edu
 
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John J Lemasters, MD, PhD

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Research Interest:

My research interests concern the cellular and molecular mechanisms underlying hypoxic and toxic injury to liver and heart cells and organs stored for transplantation surgery. In particular, my laboratory is applying new techniques of laser scanning confocal and multiphoton microscopy to characterize the physiology of single living cells, including the assessment of ion homeostasis, mitochondrial function, electrical potentials, oxygen and nitrogen free radical formation, membrane permeability and other biochemical parameters during the pathogenesis of lethal cell injury.

Honors:
GlaxoSmithKline Endowed Chair in Advanced Cellular Technologies, SmartState Center of Economic Excellence

Positions:
Director, Cell & Molecular Imaging Shared Resource, Hollings Cancer Center, Medical University of South Carolina

Selected Publications:

1.Galluzzi L, Bravo-San Pedro JM, Vitale I, Aaronson SA, Abrams JM, Adam D, Alnemri ES, Altucci L, Andrews D, Annicchiarico-Petruzzelli M, Baehrecke EH, Bazan NG, Bertrand MJ, Bianchi K, Blagosklonny MV, Blomgren K, Borner C, Bredesen DE, Brenner C, Campanella M, Candi E, Cecconi F, Chan FK, Chandel NS, Cheng EH, Chipuk JE, Cidlowski JA, Ciechanover A, Dawson TM, Dawson VL, De Laurenzi V, De Maria R, Debatin KM, Di Daniele N, Dixit VM, Dynlacht BD, El-Deiry WS, Fimia GM, Flavell RA, Fulda S, Garrido C, Gougeon ML, Green DR, Gronemeyer H, Hajnoczky G, Hardwick JM, Hengartner MO, Ichijo H, Joseph B, Jost PJ, Kaufmann T, Kepp O, Klionsky DJ, Knight RA, Kumar S, Lemasters JJ, Levine B, Linkermann A, Lipton SA, Lockshin RA, López-Otín C, Lugli E, Madeo F, Malorni W, Marine JC, Martin SJ, Martinou JC, Medema JP, Meier P, Melino S, Mizushima N, Moll U, Muñoz-Pinedo C, Nuñez G, Oberst A, Panaretakis T, Penninger JM, Peter ME, Piacentini M, Pinton P, Prehn JH, Puthalakath H, Rabinovich GA, Ravichandran KS, Rizzuto R, Rodrigues CM, Rubinsztein DC, Rudel T, Shi Y, Simon HU, Stockwell BR, Szabadkai G, Tait SW, Tang HL, Tavernarakis N, Tsujimoto Y, Vanden Berghe T, Vandenabeele P, Villunger A, Wagner EF, Walczak H, White E, Wood WG, Yuan J, Zakeri Z, Zhivotovsky B, Melino G, Kroemer G. Essential versus accessory aspects of cell death: recommendations of the NCCD 2015. Cell Death Differ. .22(1):58-73, 2014.
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2.Maldonado EN, Lemasters JJ. ATP/ADP ratio, the missed connection between mitochondria and the Warburg effect. Mitochondrion. .19 Pt A:78-84, 2014. PMCID: PMC4254332
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3.Kholmukhamedov A, Czerny C, Hu J, Schwartz J, Zhong Z, Lemasters JJ. Minocycline and doxycycline, but not tetracycline, mitigate liver and kidney injury after hemorrhagic shock/resuscitation. Shock. .42(3):256-63, 2014. PMCID: PMC4134396
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4.Qanungo S, Uys JD, Manevich Y, Distler AM, Shaner B, Hill EG, Mieyal JJ, Lemasters JJ, Townsend DM, Nieminen AL. N-acetyl-l-cysteine sensitizes pancreatic cancers to gemcitabine by targeting the NF?B pathway. Biomed Pharmacother. .68(7):855-64, 2014.
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5.Azarashvili T, Baburina Y, Grachev D, Krestinina O, Papadopoulos V, Lemasters JJ, Odinokova I, Reiser G. Carbenoxolone induces permeability transition pore opening in rat mitochondria via the translocator protein TSPO and connexin43. Arch Biochem Biophys. .558:87-94, 2014.
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6.Lemasters JJ. Variants of mitochondrial autophagy: Types 1 and 2 mitophagy and micromitophagy (Type 3). Redox Biol. .2:749-54, 2014. PMCID: PMC4085350
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7.Beck Gooz M, Maldonado EN, Dang Y, Amria MY, Higashiyama S, Abboud HE, Lemasters JJ, Bell PD. ADAM17 promotes proliferation of collecting duct kidney epithelial cells through ERK activation and increased glycolysis in polycystic kidney disease. Am J Physiol Renal Physiol. .307(5):F551-9, 2014. PMCID: PMC4154111
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8.Zhong Z, Ramshesh VK, Rehman H, Liu Q, Theruvath TP, Krishnasamy Y, Lemasters JJ. Acute ethanol causes hepatic mitochondrial depolarization in mice: role of ethanol metabolism. PLoS One. .9(3):e91308, 2014. PMCID: PMC3950152
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9.Kholmukhamedov A, Spinale FG, Mukherjee R, Lemasters JJ. Reply to "letter to the editor: 'cyclosporin A in left ventricular remodeling after myocardial infarction'". Am J Physiol Heart Circ Physiol. .306(5):H778-9, 2014.
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10.Zhang X, Lemasters JJ. Translocation of iron from lysosomes to mitochondria during ischemia predisposes to injury after reperfusion in rat hepatocytes. Free Radic Biol Med. .63:243-53, 2013. PMCID: PMC3932485
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11.Maldonado EN, Sheldon KL, DeHart DN, Patnaik J, Manevich Y, Townsend DM, Bezrukov SM, Rostovtseva TK, Lemasters JJ. Voltage-dependent anion channels modulate mitochondrial metabolism in cancer cells: regulation by free tubulin and erastin. J Biol Chem. .288(17):11920-9, 2013. PMCID: PMC3636879
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12.Hung HI, Schwartz JM, Maldonado EN, Lemasters JJ, Nieminen AL. Mitoferrin-2-dependent mitochondrial iron uptake sensitizes human head and neck squamous carcinoma cells to photodynamic therapy. J Biol Chem. .288(1):677-86, 2012. PMCID: PMC3537066
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13.Sentelle RD, Senkal CE, Jiang W, Ponnusamy S, Gencer S, Selvam SP, Ramshesh VK, Peterson YK, Lemasters JJ, Szulc ZM, Bielawski J, Ogretmen B. Ceramide targets autophagosomes to mitochondria and induces lethal mitophagy. Nat Chem Biol. .8(10):831-8, 2012. PMCID: PMC3689583
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14.Maldonado EN, Lemasters JJ. Warburg revisited: regulation of mitochondrial metabolism by voltage-dependent anion channels in cancer cells. J Pharmacol Exp Ther. .342(3):637-41, 2012. PMCID: PMC3422523
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15.Czerny C, Kholmukhamedov A, Theruvath TP, Maldonado EN, Ramshesh VK, Lehnert M, Marzi I, Zhong Z, Lemasters JJ. Minocycline decreases liver injury after hemorrhagic shock and resuscitation in mice. HPB Surg. .2012:259512, 2012. PMCID: PMC3375163
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16.Liu Q, Rehman H, Krishnasamy Y, Ramshesh VK, Theruvath TP, Chavin KD, Schnellmann RG, Lemasters JJ, Zhong Z. Role of inducible nitric oxide synthase in mitochondrial depolarization and graft injury after transplantation of fatty livers. Free Radic Biol Med. .53(2):250-9, 2012. PMCID: PMC3392495
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17.Saggu S, Hung HI, Quiogue G, Lemasters JJ, Nieminen AL. Lysosomal signaling enhances mitochondria-mediated photodynamic therapy in A431 cancer cells: role of iron. Photochem Photobiol. .88(2):461-8, 2012.
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18.Kim JS, Wang JH, Lemasters JJ. Mitochondrial permeability transition in rat hepatocytes after anoxia/reoxygenation: role of Ca2+-dependent mitochondrial formation of reactive oxygen species. Am J Physiol Gastrointest Liver Physiol. .302(7):G723-31, 2012. PMCID: PMC3330780
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19.Ramshesh VK, Lemasters JJ. Imaging of mitochondrial pH using SNARF-1. Methods Mol Biol. .810:243-8, 2012. PMCID: PMC4082984
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20.Lemasters JJ, Holmuhamedov EL, Czerny C, Zhong Z, Maldonado EN. Regulation of mitochondrial function by voltage dependent anion channels in ethanol metabolism and the Warburg effect. Biochim Biophys Acta. .1818(6):1536-44, 2011. PMCID: PMC3422743
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21.Sheldon KL, Maldonado EN, Lemasters JJ, Rostovtseva TK, Bezrukov SM. Phosphorylation of voltage-dependent anion channel by serine/threonine kinases governs its interaction with tubulin. PLoS One. .6(10):e25539, 2011. PMCID: PMC3192757
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22.Koch DG, Bogatkevich G, Ramshesh V, Lemasters JJ, Uflacker R, Reuben A. Elevated levels of endothelin-1 in hepatic venous blood are associated with intrapulmonary vasodilatation in humans. Dig Dis Sci. .57(2):516-23, 2011.
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23.Shi Y, Rehman H, Ramshesh VK, Schwartz J, Liu Q, Krishnasamy Y, Zhang X, Lemasters JJ, Smith CD, Zhong Z. Sphingosine kinase-2 inhibition improves mitochondrial function and survival after hepatic ischemia-reperfusion. J Hepatol. .56(1):137-45, 2011. PMCID: PMC3220779
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24.Rehman H, Sun J, Shi Y, Ramshesh VK, Liu Q, Currin RT, Lemasters JJ, Zhong Z. NIM811 prevents mitochondrial dysfunction, attenuates liver injury, and stimulates liver regeneration after massive hepatectomy. Transplantation. .91(4):406-12, 2011. PMCID: PMC3399729
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25.Maldonado EN, Patnaik J, Mullins MR, Lemasters JJ. Free tubulin modulates mitochondrial membrane potential in cancer cells. Cancer Res. .70(24):10192-201, 2010. PMCID: PMC3010233
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26.Kim I, Lemasters JJ. Mitochondrial degradation by autophagy (mitophagy) in GFP-LC3 transgenic hepatocytes during nutrient deprivation. Am J Physiol Cell Physiol. .300(2):C308-17, 2010. PMCID: PMC3043636
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27.Pediaditakis P, Kim JS, He L, Zhang X, Graves LM, Lemasters JJ. Inhibition of the mitochondrial permeability transition by protein kinase A in rat liver mitochondria and hepatocytes. Biochem J. .431(3):411-21, 2010. PMCID: PMC3501339
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28.Kon K, Kim JS, Uchiyama A, Jaeschke H, Lemasters JJ. Lysosomal iron mobilization and induction of the mitochondrial permeability transition in acetaminophen-induced toxicity to mouse hepatocytes. Toxicol Sci. .117(1):101-8, 2010. PMCID: PMC2923283
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29.Quiogue G, Saggu S, Hung HI, Kenney ME, Oleinick NL, Lemasters JJ, Nieminen AL. Signaling From Lysosomes Enhances Mitochondria-Mediated Photodynamic Therapy In Cancer Cells. Proc Soc Photo Opt Instrum Eng. .7380(73800C):1-8, 2009. PMCID: PMC2836728
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30.Lemasters JJ, Theruvath TP, Zhong Z, Nieminen AL. Mitochondrial calcium and the permeability transition in cell death. Biochim Biophys Acta. .1787(11):1395-401, 2009. PMCID: PMC2730424
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31.Holmuhamedov E, Lemasters JJ. Ethanol exposure decreases mitochondrial outer membrane permeability in cultured rat hepatocytes. Arch Biochem Biophys. .481(2):226-33, 2008. PMCID: PMC2656607
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32.Bajt ML, Farhood A, Lemasters JJ, Jaeschke H. Mitochondrial bax translocation accelerates DNA fragmentation and cell necrosis in a murine model of acetaminophen hepatotoxicity. J Pharmacol Exp Ther. .324(1):8-14, 2007.
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33.Hammond LE, Albright CD, He L, Rusyn I, Watkins SM, Doughman SD, Lemasters JJ, Coleman RA. Increased oxidative stress is associated with balanced increases in hepatocyte apoptosis and proliferation in glycerol-3-phosphate acyltransferase-1 deficient mice. Exp Mol Pathol. .82(2):210-9, 2006. PMCID: PMC1865130
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34.Cover C, Mansouri A, Knight TR, Bajt ML, Lemasters JJ, Pessayre D, Jaeschke H. Peroxynitrite-induced mitochondrial and endonuclease-mediated nuclear DNA damage in acetaminophen hepatotoxicity. J Pharmacol Exp Ther. .315(2):879-87, 2005.
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35.Rodriguez-Enriquez S, He L, Lemasters JJ. Role of mitochondrial permeability transition pores in mitochondrial autophagy. Int J Biochem Cell Biol. .36(12):2463-72, 2004.
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36.Schwabe RF, Uchinami H, Qian T, Bennett BL, Lemasters JJ, Brenner DA. Differential requirement for c-Jun NH2-terminal kinase in TNFalpha- and Fas-mediated apoptosis in hepatocytes. FASEB J. .18(6):720-2, 2004.
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