Research Interest:
Homeobox genes involved in the control of embryonic development. We use the mouse as a model system and gene targeting and knockout mice to study these genes. We have shown the Evxl homeobox gene to be essential for the development of all embryonic and extraembryonic tissues after implantation in the uterus. More subtle mutations in the Evxl gene are being engineered to study this gene's later roles in gastrulation and limb bud development. Through these studies we hope to further elucidate the hierarchy of molecular-genetic interactions that control development.
Positions: Director, Gene Targeting & Knockout Facility, Hollings Cancer Center, Medical University of South Carolina
Selected Publications:
| 1. | Cryopreservation and in vitro culture of primary cell types from lung tissue of a stranded pygmy sperm whale (Kogia breviceps). Comp Biochem Physiol C Toxicol Pharmacol. .155(1):136-42, 2011. PMCID: PMC3158290
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| | 2. | Moussa O, LaRue AC, Abangan RS, Williams CR, Zhang XK, Masuya M, Gong YZ, Spyropoulos DD, Ogawa M, Gilkeson G, Watson DK. Thrombocytopenia in mice lacking the carboxy-terminal regulatory domain of the Ets transcription factor Fli1. Mol Cell Biol. .30(21):5194-206, 2010. PMCID: PMC2953061
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| | 3. | Grek CL, Newton DA, Spyropoulos DD, Baatz JE. Hypoxia up-regulates expression of hemoglobin in alveolar epithelial cells. Am J Respir Cell Mol Biol. .44(4):439-47, 2010. PMCID: PMC3095918
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| | 4. | Grek CL, Newton DA, Qiu Y, Wen X, Spyropoulos DD, Baatz JE. Characterization of alveolar epithelial cells cultured in semipermeable hollow fibers. Exp Lung Res. .35(2):155-74, 2009. PMCID: PMC3366342
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| | 5. | Ellett JD, Evans ZP, Zhang G, Chavin KD, Spyropoulos DD. A rapid PCR-based method for the identification of ob mutant mice. Obesity (Silver Spring). .17(2):402-4, 2008.
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| | 6. | Zhang XK, Moussa O, LaRue A, Bradshaw S, Molano I, Spyropoulos DD, Gilkeson GS, Watson DK. The transcription factor Fli-1 modulates marginal zone and follicular B cell development in mice. J Immunol. .181(3):1644-54, 2008. PMCID: PMC2504761
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| | 7. | McCabe CD, Spyropoulos DD, Martin D, Moreno CS. Genome-wide analysis of the homeobox C6 transcriptional network in prostate cancer. Cancer Res. .68(6):1988-96, 2008. PMCID: PMC2584783
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| | 8. | Higuchi T, Bartel FO, Masuya M, Deguchi T, Henderson KW, Li R, Muise-Helmericks RC, Kern MJ, Watson DK, Spyropoulos DD. Thymomegaly, microsplenia, and defective homeostatic proliferation of peripheral lymphocytes in p51-Ets1 isoform-specific null mice. Mol Cell Biol. .27(9):3353-66, 2007. PMCID: PMC1899970
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| | 9. | Schweinfest CW, Spyropoulos DD, Henderson KW, Kim JH, Chapman JM, Barone S, Worrell RT, Wang Z, Soleimani M. slc26a3 (dra)-deficient mice display chloride-losing diarrhea, enhanced colonic proliferation, and distinct up-regulation of ion transporters in the colon. J Biol Chem. .281(49):37962-71, 2006.
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| | 10. | Smith BT, Mussell JC, Fleming PA, Barth JL, Spyropoulos DD, Cooley MA, Drake CJ, Argraves WS. Targeted disruption of cubilin reveals essential developmental roles in the structure and function of endoderm and in somite formation. BMC Dev Biol. .6:30, 2006. PMCID: PMC1533814
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| | 11. | Burger AM, Leyland-Jones B, Banerjee K, Spyropoulos DD, Seth AK. Essential roles of IGFBP-3 and IGFBP-rP1 in breast cancer. Eur J Cancer. .41(11):1515-27, 2005.
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| | 12. | Mitas M, Almeida JS, Mikhitarian K, Gillanders WE, Lewin DN, Spyropoulos DD, Hoover L, Graham A, Glenn T, King P, Cole DJ, Hawes R, Reed CE, Hoffman BJ. Accurate discrimination of Barrett's esophagus and esophageal adenocarcinoma using a quantitative three-tiered algorithm and multimarker real-time reverse transcription-PCR. Clin Cancer Res. .11(6):2205-14, 2005.
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| | 13. | Grund EM, Spyropoulos DD, Watson DK, Muise-Helmericks RC. Interleukins 2 and 15 regulate Ets1 expression via ERK1/2 and MNK1 in human natural killer cells. J Biol Chem. .280(6):4772-8, 2004.
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| | 14. | Zhang XK, Gallant S, Molano I, Moussa OM, Ruiz P, Spyropoulos DD, Watson DK, Gilkeson G. Decreased expression of the Ets family transcription factor Fli-1 markedly prolongs survival and significantly reduces renal disease in MRL/lpr mice. J Immunol. .173(10):6481-9, 2004.
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| | 15. | Masuya M, Moussa O, Abe T, Deguchi T, Higuchi T, Ebihara Y, Spyropoulos DD, Watson DK, Ogawa M. Dysregulation of granulocyte, erythrocyte, and NK cell lineages in Fli-1 gene-targeted mice. Blood. .105(1):95-102, 2004.
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| | 16. | Spyropoulos DD, Bartel FO, Higuchi T, Deguchi T, Ogawa M, Watson DK. Marker-assisted study of genetic background and gene-targeted locus modifiers in lymphopoietic phenotypes. Anticancer Res. .23(3A):2015-26, 2003.
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| | 17. | Maroulakou IG, Spyropoulos DD. The study of HOX gene function in hematopoietic, breast and lung carcinogenesis. Anticancer Res. .23(3A):2101-10, 2003.
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| | 18. | Kawada H, Ito T, Pharr PN, Spyropoulos DD, Watson DK, Ogawa M. Defective megakaryopoiesis and abnormal erythroid development in Fli-1 gene-targeted mice. Int J Hematol. .73(4):463-8, 2001.
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| | 19. | Bartel FO, Higuchi T, Spyropoulos DD. Mouse models in the study of the Ets family of transcription factors. Oncogene. .19(55):6443-54, 2000.
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| | 20. | Garcia-Gasca A, Spyropoulos DD. Differential mammary morphogenesis along the anteroposterior axis in Hoxc6 gene targeted mice. Dev Dyn. .219(2):261-76, 2000.
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| | 21. | Rogner UC, Spyropoulos DD, Le Novère N, Changeux JP, Avner P. Control of neurulation by the nucleosome assembly protein-1-like 2. Nat Genet. .25(4):431-5, 2000.
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| | 22. | Spyropoulos DD, Pharr PN, Lavenburg KR, Jackers P, Papas TS, Ogawa M, Watson DK. Hemorrhage, impaired hematopoiesis, and lethality in mouse embryos carrying a targeted disruption of the Fli1 transcription factor. Mol Cell Biol. .20(15):5643-52, 2000. PMCID: PMC86032
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| | 23. | de la Cruz CC, Der-Avakian A, Spyropoulos DD, Tieu DD, Carpenter EM. Targeted disruption of Hoxd9 and Hoxd10 alters locomotor behavior, vertebral identity, and peripheral nervous system development. Dev Biol. .216(2):595-610, 1999.
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| | 24. | Papas TS, Bhat NK, Spyropoulos DD, Mjaatvedt AE, Vournakis J, Seth A, Watson DK. Functional relationships among ETS gene family members. Leukemia. .11 Suppl 3:557-66, 1997.
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| | 25. | Spyropoulos DD, Capecchi MR. Targeted disruption of the even-skipped gene, evx1, causes early postimplantation lethality of the mouse conceptus. Genes Dev. .8(16):1949-61, 1994.
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| | 26. | Spyropoulos DD, Stallard V, Roberts BE, Cohen LK. Utilization of DNA recombination for the two-step replacement of growth factor sequences in the vaccinia virus genome. J Virol. .65(9):4609-18, 1991. PMCID: PMC248915
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| | 27. | Spyropoulos DD, Roberts BE, Panicali DL, Cohen LK. Delineation of the viral products of recombination in vaccinia virus-infected cells. J Virol. .62(3):1046-54, 1988. PMCID: PMC253665
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